Fabrication and DOE Optimization of Electrospun Chitosan/Gelatin/PVA Nanofibers for Skin Tissue Engineering

Chitosan/gelatin-based nanofibers display excellent biological performance in tissue engineering because of their biocompatible composition and nanofibrous structure with a high surface-to-volume ratio mimicking the native extracellular matrix. In this study, to save time cost experiments, response surface methodology based on Box–Behnken design (BBD) is developed predict mean diameter (chitosan:gelatin)/poly(vinyl alcohol) (PVA) three volume ratios chitosan:gelatin by considering PVA percentage, applied voltage, flow rate as input variables. The morphology chemical are investigated through scanning electron microscopy (SEM) Fourier transform infrared spectroscopy (FTIR), respectively. optimum conditions yield minimum 25:75, 50:50, 75:25 found result 165, 121, 92 nm, respectively, which show good accordance BBD estimated results. tensile testing indicates that containing higher stress lower toughness strain. water contact angle analysis (WCA) shows appropriate hydrophilicity crosslinked nanofibers. MTT assay cell viability attachment for mouse fibroblast (L929) cells. results indicate potent candidates wound healing applications.